Post tagged: histone modifications

by Rob Beagrie
in misc

tagged Enhancers, Journal Club, CRISPR-Cas, Lsd1, gene expression, histone modifications

Enhancer Journal Club: Functional annotation of native enhancers with a Cas9-histone demethylase fusion

I really enjoyed Eric Mendenhall's 2013 paper in Nature Biotechnology1, where he was able to target LSD1 histone demethylase activity to endogenous enhancers using TALE fusion proteins (read my thoughts on that paper here). So I was very interested to see a similar approach published earlier this year in …

by Rob Beagrie
in misc

tagged Enhancers, Journal Club, gene expression, histone modifications

Enhancer Journal Club: Locus-specific editing of histone modifications at endogenous enhancers.

Moving away from enhancer/promoter looping for a little while, I came across this nice paper from the Bernstein lab at the Howard Hughes Medical Institute. This paper is about assigning enhancers to their cognate genes, and also has some interesting implications for the causality of histone modifications on active …